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1K DNA Marker 

1K DNA Marker is a premixed, ready-to-load molecular weight marker containing bromophenol blue as a tracking dye.
Cat. No. Size Price Quantity  
TB40001A 500 μl USD 130
TB40001B 5 x 500 μl USD 500
Tel: +1 (973) 789-8318Email: [email protected]

Description

1K DNA Marker is a premixed, ready-to-load molecular weight marker containing six individual DNA fragments (in base pairs): 100, 300, 500, 700, 900, 1200 bp.
with 700 bp as reference indicator.

Storage Buffer

10 mM Tris-HCl (pH 7.6), 10 mM EDTA,  0.033% bromophenol blue, 0.008% xylene cyanol FF and 10% glycerol.

Protocol

1. DO NOT heat before loading.

2. Load 6 μl of 1K DNA Marker to 5 mm width lane (1.2 μl per 1 mm lane) on agarose gel or non-denaturing PAGE.

3. Following electrophoretic separation on gels ,visualize the DNA bands by ethidium bromide staining.

4. To get desired photo you can visualize the DNA bands by ethidium bromide staining after electrophoresis. 

5. Qualified agarose and fresh TAE (TBE) buffer is essential to the good photo. 

6. Use 1.5%-2.5% agarose gels for good banding. Voltage: 90-120 V.

Notes

① 1K DNA Marker is stable for at least 6 months at 4°C, and 2 years at −20°C.If stored at –20°C, mix well after thawing.

② Not designed for DNA quantification.

③ Not designed for denaturing PAGE.

④ The 100 bp band will be faint after long time electrophoresis.

6 μl 1K DNA Marker
visualized by ethidium
bromide staining on a
2.0% TAE agarose gel.
Voltage: 120 V.
TonkBio 1kb DNA Marker














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