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Taq PCR Master Mix (2x) 

2×Taq PCR Master Mix is an optimal 2× concentrated solution of Taq DNA polymerase, dNTPs, MgCl2 and all other components required for PCR, except DNA template and primers.
Cat. No. Size Price Quantity  
TB20004A 5x1 mL USD 109
TB20004B 25x1 mL USD 382
Tel: +1 (973) 789-8318Email: [email protected]

Components

2×Taq PCR Buffer
0.2 U/μl Taq DNA Polymerase
0.6 mM each dNTPs
6 mM MgCl2
PCR enhance

Overview

2×Taq PCR Master Mix is an optimal 2× concentrated solution of Taq DNA polymerase, dNTPs, MgCl2 and all other components required for PCR, except DNA template and primers. This is a premixed, ready-to-use solution with high sensitivity and specificity. The PCR products generated using this product contain dA overhangs at the 3’-end, and can be ligated to dT/-overhang vectors easily.

Applications

1) Routine PCR with high reproducibility
2) TA cloning for short fragment

Storage & Stability

Product may be stored for one month at 4 °C or 12 months at -20 °C.

Protocol

1. Thaw the 2× Taq PCR Master Mix at room temperature. Vortex and spin it briefly in a microcentrifuge to collect the material in the bottom of the tube.
 
2. Place a PCR tube on ice and add the following components for each reaction:
2×Taq PCR Master Mix 25 μl
Forward Primer (10 μM) 1 μl
Reverse Primer (10 μM) 1 μl
Template DNA *
ddH2O To 50 μl
  • *Template DNA final concentration should be no more than 10 ng/μl

Note: prepare the reaction with high precision and accuracy pipette.

 
3. Gently vortex the samples and spin down.
 
4. Perform PCR with a thermal cycler containing a heated lid using the recommended thermal cycling conditions outlined below:
Temperature/Step Time Number of Cycles
95 °C Initial Denaturation 2 min 1 Cycle
95 °C Denaturation 30 sec 30~35 cycles
55 °C Annealing※ 30 sec
72 °C Extension△ 1 min
72 °C Final Extension 5 min 1 Cycle
  • ※Approximately 5 °C below Tm of primers
  • △Extension time:approximately 1 min/kb
5.After amplification, samples can be used for agarose gel electrophoresis or be stored overnight at 2-8 °C, at -20 °C for longer storage.

Datasheet Download

Taq PCR Master Mix

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