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PCR/qPCR Related Mixes

Catalog No. Product Name Brief Description
TB20001 Golden qPCR SYBR Green Master Mix (2x) Golden qPCR SYBR Green Master Mix(2×) is an optimal 2× concentrated solution of hot-start DNA polymerase, MgCl2, dNTPs, SYBR Green I dye and all other components required for quantitative real-time PCR, except DNA template and primers.
TB20002 Golden qPCR TaqMan Probe Master Mix (2x) Golden qPCR TaqMan Probe Master Mix(2×)is a special mixture for Taqman probe real time PCR Which includes the hot-start DNA polymerase, PCR Buffer, MgCl2, dNTPs and all other components required for quantitative real-time PCR, except DNA template, primers and probes.
TB20003 Golden qPCR EVA Green Master Mix (2x) (Dye-Based) Golden qPCR EVA Green Master Mix(2×) is an optimal 2× concentrated solution of hot-start DNA polymerase, dNTPs, EVA Green dye, ROX and all other components required for quantitative real-time PCR, except DNA template and primers.
TB20004 Taq PCR Master Mix (2x) 2×Taq PCR Master Mix is an optimal 2× concentrated solution of Taq DNA polymerase, dNTPs, MgCl2 and all other components required for PCR, except DNA template and primers.

RT-PCR

Catalog No. Product Name Brief Description
TB30001 TonkBio™ First Chain cDNA Synthesis Kit The TonkBio™ First Chain cDNA Synthesis Kit for RT-PCR is optimized to efficiently synthesize first cDNA from mRNA or total RNA templates.
TB30003 TonkBio™ RT Reagent Kit (for qPCR) The TonkBio™ RT reagent Kit is designed for the first step of RT-PCR. TonkBio™ M-MLV with high extension ability can complete cDNA synthesis in 15 minutes. This kit is suitable for high throughput analysis because its easy operation and short time consumed.

Gene Recombination / Cloning

Catalog No. Product Name Brief Description
TB10001 Taq DNA Polymerase Taq DNA Polymerase is a 94 kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus (Taq) DNA polymerase gene in E. coli.
TB10002 Golden Taq DNA Polymerase Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity and a 5´ flap endonuclease activity.
TB10003 Uracil-DNA Glycosylase (UDG) The TonkBio Uracil-DNA Glycocasylase (UDG) is expressed from recombinant clone of E. Coil, and the molecular weight is 25 kDa. The optimum reaction temperature of UDG is 50°C.
TB10004 M-MLV Reverse Transcriptase (RNaseH-) M-MLV Reverse Transcriptase (RNaseH-) is a recombinant reverse transcriptase expressed and purified from E. Coil. This enzyme can be used in cDNA synthesis with long messenger RNA templates due to the lacking of RNase H activity by point mutation.
TB10006 T4 DNA Ligase T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.
TB10007 Sortase A Sortase refers to a group of prokaryotic transpeptidase that attaches surface proteins covalently to the cell wall existing in almost all Gram-positive bacteria. It contains two sub-groups, Sortase A and Sortase B.
TB10011 FlashPfu DNA Polymerase TonkBio FlashPfu is a DNA polymerase for high-fidelity PCR. By fusing Pfu with a high affinity double-stranded DNA binding domain, we have dramatically increased its processivity and extension rate. FlashPfu DNA polymerase is 50-fold more accurate than Taq polymerase and 6-fold more accurate than Pfu polymerase.

Nucleic Acid Extraction Kits

Catalog No. Product Name Brief Description
TB50001 Plasmid Miniprep Kit The Plasmid Minipred Kit is designed for efficient and convenient extraction of DNA.
TB50002 Universal DNA Purification Kit For the extraction of DNA fragments from agarose gels, PCR and enzymatic reactions.
TB50003 Bacteria Genomic DNA Extraction Kit Bacteria Genomic DNA Extraction Kit is designed for the rapid preparation of 5-20 µg genomic DNA from 109~2×109 viable bacterial cells (0.5 - 2.0 mL of culture).
TB50004 Genomic DNA Extraction Kit This kit is designed for the rapid preparation of genomic DNA from various sources including: cultured cell,tissue,blood etc. It is designed for the rapid preparation of 3-20 μg DNA from various sources.
TB50005 Blood Genomic DNA Extraction Kit Blood DNA Extraction Kit is designed for the rapid preparation of 3-20 µg genomic DNA from 100 μl-1 ml whole blood.
TB50007 Endo-Free Plasmid Maxiprep Kit The TonkBio Endo-free Plasmid Maxi Kit is designed for efficient and convenient extraction of endo-free DNA. This system applies salt free reagent to get high pure plasimd, no salt remain, and the concentration of endotoxin below 0.1 EU/μg.
TB50008 Endo-Free Plasmid Midiprep Kit The TonkBio Endo-free Plasmid Midi Kit is designed for efficient and convenient extraction of endo-free DNA. This system applies salt free reagent to get high pure plasmid, no salt remain, and the concentration of endotoxin below 0.1 EU/μg.
TB50009 Endo-Free Plasmid Miniprep Kit The TonkBio Endo-free Plasmid Mini Kit is designed for efficient and convenient extraction of endo-free DNA. This system applies salt free reagent to get high pure plasimd, no salt remain, and the concentration of endotoxin below 0.1 EU/μg.

Markers & Ladders

Catalog No. Product Name Brief Description
TB40002 2K DNA Marker 2K DNA Marker is a premixed, ready-to-load molecular weight marker containing bromophenol blue as a tracking dye.
TB40001 1K DNA Marker 1K DNA Marker is a premixed, ready-to-load molecular weight marker containing bromophenol blue as a tracking dye.
TB40004 Unstained Protein MW Marker (Low) Low Molecular Weight Protein Marker is a mixture of six purified proteins (14 to 94 kDa).
TB40003 10K DNA Marker 10K DNA Marker is a premixed, ready-to-load molecular weight marker containing bromophenol blue as a tracking dye.